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Related Experiment Videos

Human sulfate kinetics.

L John Hoffer1, Mazen J Hamadeh, Line Robitaille

  • 1Lady Davis Institute for Medical Research, Jewish General Hospital, 3755 Cote-Ste-Catherine Road, Montreal, Quebec, Canada H3T 1E2. l.hoffer@mcgill.ca

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
|July 30, 2005
PubMed
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This study reveals that human whole body sulfation is 27% of inorganic sulfate turnover. Extracellular inorganic sulfate is crucial for intracellular sulfation, with newly formed sulfate esters rapidly excreted in urine.

Area of Science:

  • Biochemistry
  • Physiology
  • Metabolic Research

Background:

  • Sulfate metabolism is vital for numerous biological processes, including detoxification and biosynthesis.
  • Understanding the kinetics of inorganic sulfate and sulfate esters is crucial for metabolic research.

Purpose of the Study:

  • To quantify whole-body inorganic sulfate turnover and sulfation rates in humans.
  • To investigate the kinetic profiles of inorganic sulfate and sulfate esters.
  • To elucidate the relationship between extracellular inorganic sulfate and intracellular sulfation.

Main Methods:

  • Utilized stable isotope tracer techniques with sodium [34S]sulfate.
  • Employed electrospray tandem mass spectrometry for precise measurements.
  • Analyzed serum and urinary inorganic sulfate and sulfate ester kinetic profiles.

Related Experiment Videos

  • Applied compartmental modeling to link inorganic sulfate and sulfate ester kinetics.

    • Main Results:

    • Whole body inorganic sulfate turnover was 841 +/- 49 micromol/h.
    • Whole body sulfation rate was determined to be 232 +/- 36 micromol/h, representing 27% of sulfate turnover.
    • Sulfate esters appeared in serum and urine within 1 hour of tracer administration.
    • Approximately 50% of the total sulfation rate was attributed to sulfate ester appearance and excretion.

    Conclusions:

    • Human whole body sulfation represents a significant portion of inorganic sulfate turnover.
    • Extracellular inorganic sulfate serves as a key reservoir for intracellular sulfation processes.
    • Newly synthesized sulfate esters are efficiently released into the extracellular space for urinary excretion.