Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A dual-frequency QCM-D setup operating at elevated oscillation amplitudes.

Malin Edvardsson1, Michael Rodahl, Bengt Kasemo

  • 1Department of Applied Physics, Chalmers University of Technology, SE-412 96 Göteborg, Sweden. maline@fy.chalmers.se

Analytical Chemistry
|August 2, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Impact of Polyethylene Glycol Lipid Anchors on the Physicochemical Properties, Protein Corona, Function, and Biodistribution of Lipid Nanoparticles.

ACS nano·2026
Same author

Effects of Serum Incubation on Lipid Nanoparticle PEG Shedding, mRNA Retention, and Membrane Interactions.

ACS applied materials & interfaces·2025
Same author

Quantitative Detection of Biological Nanoparticles Using Twilight Off-Axis Holographic Microscopy: Insights on Complex Formation between PEGylated Gold Nanoparticles and Lipid Vesicles.

The journal of physical chemistry. B·2025
Same author

Image analysis optimization for nanowire-based optical detection of molecules.

Nanophotonics (Berlin, Germany)·2025
Same author

Multiparametric functional characterization of individual lipid nanoparticles using surface-sensitive light-scattering microscopy.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Optical label-free microscopy characterization of dielectric nanoparticles.

Nanoscale·2025

The quartz crystal microbalance with dissipation monitoring (QCM-D) technique was enhanced for dual-frequency operation. Elevated oscillation amplitudes influenced lipid bilayer formation but not streptavidin binding to monomers, though it inhibited binding to larger particles.

Area of Science:

  • Biomolecular interaction analysis
  • Surface science
  • Analytical chemistry

Background:

  • The quartz crystal microbalance (QCM) technique's application is questioned regarding oscillation effects on adsorption kinetics.
  • Elevated oscillation amplitudes may influence biomolecular interactions.

Purpose of the Study:

  • To develop and evaluate a dual-frequency quartz crystal microbalance with dissipation monitoring (QCM-D) technique.
  • To investigate the influence of sensor oscillation amplitude on adsorption and binding events.

Main Methods:

  • Developed a dual-frequency QCM-D mode: one harmonic for continuous excitation at variable amplitudes, another for frequency and dissipation measurements.
  • Investigated lipid vesicle adsorption and decomposition into supported lipid bilayers on SiO2.

Related Experiment Videos

  • Studied streptavidin binding to biotin-modified surfaces, including protein monomers and biotin-binding protein coupled to colloidal particles.

    • Main Results:

    • Elevated oscillation amplitudes significantly influenced lipid bilayer formation kinetics.
    • No influence of oscillation amplitude was observed on streptavidin monomer binding to biotin.
    • Binding of biotin-binding protein coupled to 200 nm colloidal particles was inhibited at elevated amplitudes.

    Conclusions:

    • The QCM-D technique's oscillation amplitude can influence certain surface-associated processes, like lipid bilayer formation.
    • The effect of oscillation amplitude is dependent on the specific biomolecular interaction and system studied.
    • Dual-frequency QCM-D offers a method to probe and potentially control biomolecular interactions by varying oscillation amplitude.