Related Experiment Video

Updated: Aug 2, 2026

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro
16:13

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Published on: June 13, 2011

The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization

K Dheda1, J F Huggett, J S Chang

  • 1Centre for Infectious Diseases and International Health, Royal Free and University College Medical School, and Department of Thoracic and HIV Medicine, Royal Free Hospital, London NW3 2QG, UK.

Analytical Biochemistry
|August 2, 2005
PubMed
Summary

No abstract available in PubMed .

More Related Videos

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
11:37

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
13:16

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

Related Experiment Videos

Last Updated: Aug 2, 2026

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro
16:13

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Published on: June 13, 2011

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
11:37

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
13:16

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

Articles linked to this work by shared authors, journal, and citation graph.

What's new in TB diagnosis? A spotlight on active case-finding.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2026

Quantifying emergent drug-resistant TB using laboratory data and record-linkage methods.

IJTLD open·2026

Clinical standards for antimicrobial stewardship in TB care.

IJTLD open·2025

South African Thoracic Society consensus statement on transbronchial lung cryobiopsy for interstitial lung disease.

African journal of thoracic and critical care medicine·2025

Drug management of TB in the intensive care setting: an international multicentre study.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2025

Feasibility and safety of transbronchial lung cryobiopsy and mediastinal lymph node cryobiopsy: Experience from a resource limited African setting.

African journal of thoracic and critical care medicine·2025

Voltammetric study of doxorubicin interaction with single-stranded and double-stranded DNA.

Analytical biochemistry·2026

A dual signal ratio electrochemical sensor based on DNA tetrahedrons and bimetallic nanocomposites for AFB1 analysis.

Analytical biochemistry·2026

An enzyme-free HCR-amplified colorimetric biosensor based on a Cu2+/rGO nanozyme for detection of dengue virus.

Analytical biochemistry·2026

Rapid identification of dried suckling deer using molecular authentication-based PCR-GICA and PCR-FQICA.

Analytical biochemistry·2026

A rapid and accurate discrimination of Hedyotis diffusa Willd. from its adulterants.

Analytical biochemistry·2026

Mitochondrial DNA digital PCR improves metastasis quantification in the chick chorioallantoic membrane (CAM) assay.

Analytical biochemistry·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us