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Published on: December 28, 2017
Genetic diversity of Histoplasma capsulatum strains in Brazil
Rosely M Zancopé-Oliveira1, Patrícia Morais e Silva Tavares, Mauro de Medeiros Muniz
1Instituto de Pesquisa Clínica Evandro Chagas, FIOCRUZ (Funda ção Oswaldo Cruz.), Rio de Janeiro, Brazil. zancope@ipec.fiocruz.br
Abstract:
This study establishes the genetic relatedness among Brazilian Histoplasma capsulatum samples obtained from different sources. A PCR-based random amplified polymorphic DNA (RAPD) assay was used to delineate polymorphisms among isolates in geographically diverse regions in Brazil. RAPD fingerprints revealed distinct DNA profiles and provided a high level of discrimination among H. capsulatum strains from different locations. Cluster I was composed of H. capsulatum isolates from the northeast region. The majority of strains from southeast and south were categorized as major cluster II. The strain 84564 from Rio de Janeiro State showed no genetic correlation to any of the isolates from the same state. The RAPD patterns of H. capsulatum isolates from Goias (Cluster III) were unrelated to DNA fingerprints observed among the other H. capsulatum strains (48% similarity). This study is the first report that stratifies the clusters of H. capsulatum strains from Brazil by molecular typing and associates them with the geographical origin.
