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Updated: Aug 16, 2026

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
Published on: February 20, 2013
Proteolysis: anytime, any place, anywhere?
Jonathon Pines1, Catherine Lindon
1Wellcome Trust/Cancer Research UK, Gurdon Institute & Department of Zoology, Tennis Court Road, Cambridge CB2 1QN, UK. jp103@cam.ac.uk
Abstract:
Proteolysis via the ubiquitin-proteasome system (UPS) is a rapid and effective method of degrading a specific protein at a specific time, and in many cases a protein is degraded only in response to a particular cellular signal or event. However, an added dimension to the control of protein degradation is possible because the ubiquitin system can be spatially regulated. Controlling where a protein is degraded can enhance the specificity and timing of proteolysis, generate asymmetry and maintain sub-compartments even in the mitotic cell. Here, we discuss this aspect of the UPS.
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