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Inactivation of enteric adenovirus and feline calicivirus by ozone

Jeanette A Thurston-Enriquez1, Charles N Haas, Joseph Jacangelo

  • 1United States Department of Agriculture-Agricultural Research Service, University of Nebraska East Campus, Lincoln, NE 68583-0934, USA. jthurston2@unl.edu

Water Research
|August 3, 2005
PubMed

Insights

Ozone effectively inactivates feline calicivirus (FCV) and adenovirus type 40 (AD40) at low concentrations. This study provides crucial data on ozone disinfection efficacy for water treatment, showing significant virus inactivation under specific conditions.

Area of Science:

  • Environmental microbiology
  • Water treatment technologies
  • Virology

Background:

  • Limited data exists on ozone's effectiveness against caliciviruses and enteric adenoviruses.
  • Feline calicivirus (FCV) serves as a surrogate for human caliciviruses.
  • Adenovirus type 40 (AD40) represents enteric adenoviruses.

Purpose of the Study:

  • To determine the efficacy of ozone in inactivating FCV and AD40.
  • To establish ozone Ct values for a 4-log reduction of these viruses.
  • To compare generated Ct values with existing regulatory guidelines.

Main Methods:

  • Virus inactivation experiments using FCV and AD40.
  • Controlled conditions: pH 7, 5°C, disinfectant demand-free water.
  • Application of the efficiency factor hom (EFH) model to determine Ct values.

Main Results:

  • Ct values for 4-log inactivation at 5°C and pH 7 ranged from 0.07 to 0.60 mg/l min for AD40 and <0.01 to 0.03 mg/l min for FCV.
  • Generated Ct values were lower than those specified by the US EPA Guidance Manual.
  • Low ozone residuals (<0.01 mg/l) demonstrated substantial inactivation of both viruses.

Conclusions:

  • Ozone is a highly effective disinfectant for FCV and AD40 at low concentrations.
  • The study provides valuable Ct values for ozone disinfection in water treatment.
  • Findings suggest ozone can meet stringent disinfection requirements for public water systems.

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