Cryopreservation of poultry sperm: the enigma of glycerol

This review summarizes recent data for cryopreservation of poultry sperm and data establishing the contraceptive effect of glycerol. Successful cryopreservation protocols for bovine sperm are compared to the requirements for rooster sperm, with emphasis on glycerol-induced alterations in avian reproductive systems. It has been shown that molar concentrations of glycerol can affect (a) physical features of the cytoplasm (cytoplasmic organization and viscosity), (b) permeability and stability of the membrane bilayer(s), and (c) noncovalent attachment of proteins to the sperm surface. Perturbing effects of glycerol on sperm metabolism and the essentiality of maintaining bioenergetic balance during the temperature changes associated with any cryopreservation protocol are discussed. Emphasis is placed on the processes in avian reproduction that may be altered by interactions with glycerol. Finally, we discuss the potential value of using available genetic models (lines of roosters differing in the capacity of their sperm to survive a freeze-thaw cycle) to clarify and overcome damage to poultry sperm induced by cryopreservation.