Calcification and stress distribution in bovine pericardial heart valves

There is a strong relationship between mechanical stress and calcification in biological prosthetic heart valves. A dynamic in vitro calcification test has been used to study the relationship between stress distributions in the leaflets of bovine pericardial valves and the deposition of calcium over the leaflet surfaces. Intuitive stress regions have been defined over the leaflet surfaces. Calcium uptake by the leaflets has been assayed directly by ashing of leaflet material and analysis of the ash by atomic absorption spectrophotometry. Calcium and phosphorus distribution over the leaflet surface has been analyzed using energy-dispersive x-ray analysis by scanning electron microscope and data points assigned to the appropriate stress region. The uptake of calcium is assessed by comparing stress regions, surfaces, and the degree of calcification of the valve. Differences between stress regions and surfaces are significant. Uptake of calcium in these valves appears to be strongly related to the degree and type of stress present in the valve leaflets.