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Sensor and biosensor preparation, optimisation and applications of Prussian Blue modified electrodes.
1Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy. francesco.ricci@uniroma2.it
Biosensors & Bioelectronics
|August 4, 2005
Summary
Prussian Blue is a versatile mediator for oxidase enzyme-based biosensors, enabling sensitive detection of analytes like glucose and cholesterol in clinical, food, and environmental samples. This review highlights its advantages, drawbacks, and commercial potential in analytical applications.
Area of Science:
- Electrochemistry
- Analytical Chemistry
- Biosensor Technology
Background:
- Prussian Blue is a widely used electrochemical mediator in analytical applications.
- Its ability to catalyze hydrogen peroxide reduction is key for oxidase enzyme-based biosensors.
Purpose of the Study:
- To review the biosensing applications of Prussian Blue.
- To provide an overview of its advantages, drawbacks, and commercial potential.
Main Methods:
- Modification of electrode surfaces with Prussian Blue.
- Detection of hydrogen peroxide at low potentials (around 0.0 V vs. Ag/AgCl).
- Coupling with oxidase enzymes for analyte detection.
Main Results:
- Prussian Blue facilitates sensitive detection of various analytes including glucose, lactate, cholesterol, and galactose.
- It enables detection in clinical, environmental, and food analysis.
- Recent trends include pesticide determination and improved food analysis methods.
Conclusions:
- Prussian Blue is a valuable mediator for developing efficient and selective biosensors.
- It offers advantages in reducing electrochemical interferences.
- Future applications and commercial potential are promising.