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Updated: Aug 11, 2026

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
Published on: December 9, 2015
[Establishment of a drug resistant leukemia cell line caused by mdr1 gene]
Ling Gu1, Ting Liu, Yu-ping Gong
1Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To establish a drug resistant leukemia cell line caused by mdr1 gene for the mdr1 reversing research.
Methods:
The leukemia sensitive cell line K562 was transfected with mdr1 cDNA by liposome-induced gene transfection and selected by G418. The mdr1 phenotype was identified by RT-PCR, the P-gp expression was detected by flow cytometry, the function of P-gp was measured by Rh123 efflux experiment, and the sensitivity of cell lines to the drug was detected by MTT test. Results The transfected K562/mdr1 with mdr1 cDNA highly expressed mdr1 and showed a remarkable drug resistant feature.
Conclusion:
mdr1 plays an important role in drug resistance and the mdr1 cell line established by gene transfection can provide a better cell model for mdr1 reversing research.
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