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Updated: Aug 16, 2026
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
Efficient transfection of DNA by liposomes formulated with cationic gemini amphiphiles
Cecilia Bombelli1, Francesca Faggioli, Paola Luciani
1Istituto di Metodologie Chimiche del CNR and Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
Abstract:
Cationic liposomes formulated with neutral 1,2-dimyristoyl-sn-glycero-3-phosphocholine and cationic gemini surfactants were used for transfecting different cell lines with a reporter gene. The efficiency in the transfection has been correlated to the high extent of DNA condensation observed by circular dichroism, condensation shown to depend heavily on the gemini spacer structure. Transfection efficiency was better than that obtained with a commercial lipofection kit.
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