Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tn5 transposase-mediated mouse transgenesis.

Ryota Suganuma1, Pawel Pelczar, Jean François Spetz

  • 1Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822, USA.

Biology of Reproduction
|August 5, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction Note: Cre/lox system to develop selectable marker free transgenic tobacco plants conferring resistance against sap sucking homopteran insect.

Plant cell reports·2025
Same author

Immunotherapy against colorectal cancer via delivery of anti-PD-L1 nanobody mRNA.

eGastroenterology·2025
Same author

Sex Determination by a Univalent Chromosome in the Rhizocephalan <i>Peltogasterella gracilis</i> (Cirripedia: Rhizocephala: Peltogasterellidae).

The Biological bulletin·2025
Same author

Interleukin-34-dependent perivascular macrophages promote vascular function in the brain.

Immunity·2025
Same author

Differences in Susceptibility to SARS-CoV-2 Infection Among Transgenic hACE2-Hamster Founder Lines.

Viruses·2024
Same author

A safety guide for transgenic Cre drivers in metabolism.

Nature metabolism·2024

We developed a new mouse transgenesis method using hyperactive Tn5 transposase for efficient DNA insertion. This technique consistently produces transgenic mice with fewer oocytes than traditional methods.

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Reproductive Biology

Background:

  • Traditional mouse transgenesis methods like pronuclear microinjection are often inefficient and require significant resources.
  • Developing more effective and consistent methods for generating transgenic mice is crucial for genetic research.

Purpose of the Study:

  • To introduce a novel, highly effective method for creating transgenic mice.
  • To leverage hyperactive Tn5 transposase for enhanced DNA integration into the mouse genome.

Main Methods:

  • Utilizing a hyperactive Tn5 transposase system for transgene insertion during intracytoplasmic sperm injection.
  • Applying the method to both hybrid and inbred mouse strains.
  • Testing the efficacy of the technique with round spermatids.

Related Experiment Videos

Main Results:

  • Achieved significantly higher integration effectiveness compared to conventional pronuclear microinjection and sperm injection methods.
  • Demonstrated consistent production of transgenic mice, requiring fewer manipulated oocytes.
  • Confirmed the effectiveness of transposase-mediated transgenesis with round spermatids.

Conclusions:

  • The novel transposase-mediated method offers a more efficient and consistent approach to mouse transgenesis.
  • This technique reduces the number of oocytes needed, optimizing resource utilization in transgenic mouse production.
  • The method holds potential for fertility rescue in azoospermic animals using sperm precursor cells.