[Computer-assisted 3-D reconstruction of biological objects using serial sections]

In this review, the literature data on the major directions and problems of experimental technology of "3-D Histology", based on the analysis of serial sections, are discussed. The main attention is drawn to the principle problem: achievement of realness (accuracy) of generated 3-D models, i. e. to the problem of correspondence of native tissue structure to its computer 3-D model. The review consecutively presents the possible sources of distortions which accompany the researchers during 3-D modeling. The artifacts are considered which arise at a stage of computer 3-D modeling on the basis of serial histological sections of investigated structures. The special attention is given to distortion of the native tissue morphology during histological processings in the course of preparation of serial sections. The aspects and problems discussed in this review, are important for the transition from 3-D tissue reconstruction to 3-D histology.