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Updated: Aug 16, 2026

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
Published on: February 26, 2010
Diffusion-influenced excited-state reversible transfer reactions, A* + Bright harpoon over left harpoonC* + D, with
Soohyung Park1, Kook Joe Shin, Alexander V Popov
1School of Chemistry, Seoul National University, Korea.
Abstract:
We report accurate Brownian simulation results for the kinetics of the pseudo-first-order diffusion-influenced excited-state reversible transfer reaction A(*) + Bright harpoon over left harpoonC(*) + D with two different lifetimes using two different propagation algorithms. The results are used to test approximate solutions for this many-particle problem. Available theories fail when one of the two reactions or (decay) rate constants is large. To remedy this situation, we develop two uniform approximations, which are based on introducing a generalized Smoluchowski term into the relaxation-time approximation. The best of these is the extended unified theory of reversible target reactions, which reduces correctly in all limits and exhibits superior agreement with simulations.
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