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Quantifying recombinant proteins and their degradation products using SDS-PAGE and scanning laser densitometry

Aaron P Miles1, Allan Saul

  • 1Biochemical Assay Development and Quality Control, Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, Rockville, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 6, 2005
PubMed
Abstract

No abstract available in PubMed .

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SDS-PAGE01:27

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Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...