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Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology
Published on: July 3, 2018
Immunohistochemical staining of epoxy resin sections of peripheral nerve
Zhao Cai1, Jim Manavis, Kathy Cash
1Centre for Neurological Disease, Hanson Institute, Adelaide, Australia.
Applied Immunohistochemistry & Molecular Morphology : AIMM
|August 6, 2005
Abstract:
The authors describe a simple and cost-effective method of immunostaining semithin epoxy resin sections of peripheral nerve for light microscopy with antibodies to myelin protein zero, peripheral myelin protein 22, myelin basic protein, and neurofilament protein 200 using a combined technique of surface etching with sodium ethoxide and heat-induced antigen retrieval.
