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Related Experiment Videos

Heterogeneous alterations in human alloimmunity associated with immunization.

Meagan Roddy1, Michael Clemente, Emilio D Poggio

  • 1Department of Immunology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA.

Transplantation
|August 6, 2005
PubMed
Summary

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Therapeutic vaccinations can non-specifically augment cellular and humoral alloimmunity in transplant candidates. Monitoring immune responses is recommended before and after immunization to manage potential impacts on graft outcomes.

Area of Science:

  • Transplantation immunology
  • Vaccinology
  • Immunogenetics

Background:

  • Pre-transplant alloimmunity (alloantibodies, alloreactive T cells) impacts graft survival.
  • Environmental factors, like therapeutic vaccinations, can modulate alloimmunity.

Purpose of the Study:

  • To prospectively evaluate the impact of two immunization protocols on human cellular and humoral alloimmunity.
  • To understand how vaccination influences pre-existing alloimmune responses.

Main Methods:

  • IFNgamma ELISPOT assay for cellular alloimmunity.
  • Flow cytometric analysis of HLA-coated beads for alloantibody reactivity.
  • Prospective evaluation of human subjects undergoing immunization.

Main Results:

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  • Over 50% of subjects showed augmented cellular and/or humoral alloimmune reactivity post-vaccination.
  • Immune response augmentation was heterogeneous, with transient and persistent effects observed.
  • No cross-reactivity between vaccine antibodies and alloantibodies suggested a nonspecific adjuvant effect.

Conclusions:

  • Therapeutic vaccinations can alter the strength of human cellular and humoral alloimmunity.
  • Serial immune monitoring of alloreactivity is advisable for potential transplant recipients receiving immunizations.