Related Experiment Video

Updated: Aug 16, 2026

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
07:15

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

Inhibition of apoptosis by Nur77 through NF-kappaB activity modulation

L de Léséleuc1, F Denis

  • 11INRS-Institut Armand-Frappier, Laval, QC, Canada.

Cell Death and Differentiation
|August 6, 2005
PubMed

Insights

The orphan nuclear receptor Nur77 enhances cell survival by resisting apoptosis through NF-kappaB signaling. This study reveals Nur77

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • The orphan nuclear receptor Nur77 plays a complex role in cell fate, with reported associations with both apoptosis promotion and resistance.
  • Understanding Nur77's precise function under various apoptotic stimuli is crucial for its therapeutic potential.

Purpose of the Study:

  • To investigate the specific role of Nur77 in mediating cellular responses to diverse apoptotic triggers.
  • To elucidate the molecular mechanisms underlying Nur77's influence on programmed cell death.

Main Methods:

  • Overexpression of Nur77 in HEK293 cells.
  • Induction of apoptosis using death receptor ligands, DNA-damaging agents, and endoplasmic reticulum stress.
  • Assessment of NF-kappaB activity and cIAP1 gene expression.
  • Use of dominant-negative Nur77 and DNA-binding inhibitors.

Main Results:

  • Nur77 overexpression conferred resistance to apoptosis induced by multiple stimuli.
  • Nur77 enhanced NF-kappaB activity, which was essential for its antiapoptotic effects, including the induction of cIAP1.
  • Dominant-negative Nur77 sensitized cells to death receptor-mediated apoptosis but protected against ER stress-induced apoptosis.

Conclusions:

  • Nur77 acts as a key regulator of apoptosis, promoting cell survival through NF-kappaB-dependent pathways.
  • The context-dependent effects of Nur77 highlight intricate nuclear crosstalk influencing cell fate decisions.
  • Nur77's dual role in apoptosis modulation offers insights into targeted cancer therapies.

More Related Videos

Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur7712:30

Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77

Published on: August 17, 2015

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity04:20

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity

Published on: February 9, 2024

Related Concept Videos

The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...