Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RNA interference against enterovirus 71 infection.

Adrian Chong Nyi Sim1, Arthur Luhur, Theresa May Chin Tan

  • 1Department of Microbiology, Faculty of Medicine, National University of Singapore, MD4A, 5 Science Drive 2, 117597, Singapore. adriansim@alumni.nus.edu.sg

Virology
|August 9, 2005
PubMed
Summary

RNA interference (RNAi) using small interfering RNA (siRNA) effectively inhibits Enterovirus 71 (EV71), a cause of hand, foot, and mouth disease. This study shows siRNA therapy is a promising, safe, and effective treatment for EV71 infections.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antiviral Strategies Targeting Enteroviruses: Current Advances and Future Directions.

Viruses·2025
Same author

Establishment of Drosophila intestinal cell lines as tools for multiomic screening and deciphering intestinal biology.

Scientific reports·2025
Same author

Correction: Revolutionizing immunization: a comprehensive review of mRNA vaccine technology and applications.

Virology journal·2025
Same author

mRNA Vaccine Development in the Fight Against Zoonotic Viral Diseases.

Viruses·2025
Same author

Revolutionizing immunization: a comprehensive review of mRNA vaccine technology and applications.

Virology journal·2025
Same author

Current status of the development of dengue vaccines.

Vaccine: X·2025

Area of Science:

  • Virology
  • Molecular Biology
  • Therapeutics

Background:

  • Enterovirus 71 (EV71) is a primary cause of hand, foot, and mouth disease (HFMD), often leading to severe neurological issues.
  • Currently, no effective antiviral therapies exist for EV71 infections.

Purpose of the Study:

  • To investigate the potential of RNA interference (RNAi) as a therapeutic strategy against EV71.
  • To evaluate the efficacy and specificity of small interfering RNAs (siRNAs) targeting different regions of the EV71 genome.

Main Methods:

  • Chemically synthesized siRNAs targeting specific EV71 genomic regions (3'UTR, 2C, 3C, 3D) were designed.
  • Rhabdomyosarcoma (RD) cells were transfected with siRNAs, followed by EV71 infection to assess viral inhibition.
  • Viral RNA levels, protein expression, and plaque formation were quantified to determine siRNA efficacy.

Related Experiment Videos

Main Results:

  • Transfection with siRNAs targeting the 3'UTR, 2C, 3C, or 3D regions significantly reduced EV71-induced cytopathic effects.
  • The antiviral effect was dose-dependent, correlating with decreased viral RNA, protein, and plaque formation.
  • siRNA-mediated inhibition persisted for at least 48 hours post-transfection with no observed off-target effects.

Conclusions:

  • RNA interference using siRNA demonstrates significant potential as a targeted antiviral therapy for Enterovirus 71.
  • siRNA therapy offers a feasible and specific approach to combat EV71 infections, warranting further clinical investigation.