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2-micron circle plasmids do not reduce yeast life span.

Alaric A Falcon1, Natalie Rios, John P Aris

  • 1Department of Anatomy and Cell Biology, Health Science Center, 1600 SW Archer Road, University of Florida, Gainesville, FL 32610-0235, USA. alaricf@stanford.edu

FEMS Microbiology Letters
|August 9, 2005
PubMed
Summary
This summary is machine-generated.

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The 2-micron plasmid in yeast does not shorten replicative life span. Accumulation of extrachromosomal DNA, not the 2-micron plasmid itself, is linked to reduced yeast lifespan.

Area of Science:

  • Cellular Biology
  • Genetics
  • Aging Research

Background:

  • Extrachromosomal rDNA circles (ERCs) and ARS-plasmids are hypothesized to shorten yeast replicative life span through asymmetric inheritance.
  • The common 2-micron plasmid in Saccharomyces cerevisiae has stable mitotic inheritance, but its effect on aging is unstudied.

Purpose of the Study:

  • To investigate whether the 2-micron plasmid impacts the replicative life span of budding yeast.
  • To determine if 2-micron circles accumulate in aged yeast cells.

Main Methods:

  • Utilized a method to remove the 2-micron plasmid from laboratory yeast strains, creating isogenic cir+ and cir0 strains.
  • Compared the replicative life spans of yeast strains with and without the 2-micron plasmid.

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Main Results:

  • No significant difference in replicative life span was observed between yeast strains with (cir+) and without (cir0) the 2-micron plasmid.
  • 2-micron circles did not show accumulation in older yeast cells.

Conclusions:

  • Naturally occurring levels of the 2-micron plasmid do not negatively affect yeast replicative life span.
  • Asymmetric inheritance and subsequent accumulation of DNA episomes, rather than the presence of the 2-micron plasmid, are critical for life span reduction.