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Updated: Aug 10, 2026

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
Published on: April 17, 2014
Design and expression of oligomeric fibronectin fusion protein: a strategy for enhancing cell adhesion activity
Chong-Pyoung Chung1, Jun-Hyeog Jang
1Intellectual Biointerface Engineering Center and Department of Periodontology, Seoul National University College of Dentistry, Korea.
Abstract:
Cell adhesion to extracellular matrices, including fibronectin, results in clustering of integrins in focal adhesions. To promote the clustering of fibronectin and thus enhance its activity at the sites of focal adhesion formation, we have engineered a fusion protein containing recombinant fibronectin fragment (hFN) connected to the tetramerization helix domain of lac repressor for oligomeric assembly. Purified Lac-hFN fusion protein exhibited significant increase of cell adhesion and proliferation of GF cells compared with hFN alone (p < 0.05).
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