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IL-10 plasma levels are elevated after LPS injection in splenectomized A/J mice
Manuel B Torres1, Virginia L Vega, Mazen Bedri
1Division of Pediatric Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
The Journal of Surgical Research
|August 10, 2005
Summary
Splenectomy (surgical removal of the spleen) impacts the inflammatory response to LPS, with effects varying by mouse genetic background. The spleen modulates inflammation beyond just clearing bacteria.
Area of Science:
- Immunology
- Sepsis Pathophysiology
- Inflammation Research
Background:
- Splenectomy is indicated for hypersplenism and trauma.
- It increases sepsis risk (overwhelming post-splenectomy sepsis - OPSS).
- The spleen's role in inflammation regulation is under investigation.
Purpose of the Study:
- To investigate the effect of splenectomy on lipopolysaccharide (LPS)-induced inflammation in mice.
- To explore the spleen's role in modulating inflammatory responses.
Main Methods:
- Male mice of different strains underwent splenectomy (SPX), sham operation (SHAM), or no operation (NoOp).
- Mice were injected with LPS, and plasma cytokine levels were measured.
- Peritoneal macrophages (PMphi) were isolated and stimulated with LPS.
Main Results:
- Splenectomized A/J and DBA/2J mice showed elevated IL-10 plasma levels post-LPS injection compared to controls.
- Elevated IL-10 production by macrophages was observed in splenectomized A/J mice.
- Responses varied across different mouse strains, indicating a genetic influence.
Conclusions:
- The spleen significantly modulates LPS-induced inflammatory processes.
- This modulatory role extends beyond the spleen's known function in pathogen clearance.
- Genetic background influences the spleen's contribution to inflammation.
