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Related Experiment Videos

Proanthocyanidins, from Ribes nigrum leaves, reduce endothelial adhesion molecules ICAM-1 and VCAM-1.

N Garbacki1, M Kinet, B Nusgens

  • 1CHU, Tour 3, Université de Liège, Avenue de l'Hôpital, 3, B-4000 Sart Tilman, Belgium. N.Garbacki@ulg.ac.be

Journal of Inflammation (London, England)
|August 11, 2005
PubMed
Summary

Blackcurrant proanthocyanidins (PACs) reduce inflammation by inhibiting leukocyte infiltration and down-regulating endothelial adhesion molecules like ICAM-1 and VCAM-1. These compounds also modulate TNF-alpha-induced VEGF transcription, supporting their anti-inflammatory effects.

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Area of Science:

  • Pharmacology
  • Immunology
  • Natural Products Chemistry

Background:

  • Investigates the anti-inflammatory effects of proanthocyanidins (PACs) from blackcurrant leaves.
  • Focuses on the impact of PACs on neutrophil accumulation in inflammatory conditions.

Purpose of the Study:

  • To evaluate the in vivo and in vitro effects of blackcurrant PACs on inflammatory processes.
  • To determine the mechanisms by which PACs exert anti-inflammatory activity, specifically regarding leukocyte infiltration and adhesion molecules.

Main Methods:

  • In vivo studies utilized carrageenan-induced pleurisy in rats, measuring exudate and PMN accumulation.
  • Flow cytometry analyzed leukocyte adhesion molecule mobilization, while immunohistochemistry detected endothelial adhesion molecules (ICAM-1, VCAM-1).

Related Experiment Videos

  • In vitro studies assessed ICAM-1, IL-8, and VEGF mRNA expression in TNF-alpha-stimulated endothelial cells treated with PACs.

    • Main Results:

    • PACs dose-dependently inhibited carrageenan-induced pleurisy, reducing exudate and PMN infiltration.
    • Endothelial cell adhesion molecule production (ICAM-1, VCAM-1) was decreased, while leukocyte adhesion molecule mobilization was unaffected.
    • In vitro, PACs significantly inhibited ICAM-1 mRNA expression and dose-dependently enhanced VEGF121 mRNA expression.

    Conclusions:

    • Blackcurrant PACs exhibit anti-inflammatory activity by inhibiting leukocyte infiltration.
    • This effect is partly mediated by down-regulating endothelial adhesion molecules ICAM-1 and VCAM-1.
    • PACs can modulate TNF-alpha-induced VEGF transcription, indicating a role in inflammatory pathway regulation.