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Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

Inherent potential for production of tumor necrosis factor-alpha by human intestinal macrophages

Kazue Nakata1, Hiroyuki Inagawa, Takashi Nishizawa

  • 1Institute for Health Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, 770-8514, Japan.

International Journal of Colorectal Disease
|August 11, 2005
PubMed
Abstract

Insights

Intestinal macrophages can produce tumor necrosis factor (TNF), a key factor in Crohn's disease (CD). Specific stimuli like Sarcophaga lectin or immunoglobulin-A coating can activate this TNF production, offering insights into CD pathogenesis.

Area of Science:

  • Immunology
  • Gastroenterology
  • Cell Biology

Background:

  • Tumor necrosis factor (TNF) is implicated in Crohn's disease (CD) pathogenesis.
  • Resident intestinal macrophages are reported to not produce TNF upon lipopolysaccharide (LPS) stimulation.
  • The inherent capacity of intestinal macrophages to produce TNF remains unproven.

Purpose of the Study:

  • To determine if intestinal macrophages possess an intrinsic potential for TNF production.
  • To investigate factors influencing TNF production by colonic macrophages.

Main Methods:

  • Colonic macrophages were isolated from human intestinal lamina propria.
  • Macrophages were stimulated with various agents including LPS, ONO-4007, OK-432, phorbol 12-myristate 13-acetate, and lectins.
  • Macrophage responses were assessed on different culture substrates (collagen vs. immunoglobulin-A coated dishes).

Main Results:

  • Colonic macrophages, negative for CD14 and positive for CD68, produced minimal TNF in response to LPS.
  • Sarcophaga lectin was the only tested substance that induced TNF production by intestinal macrophages.
  • Culture on immunoglobulin-A coated dishes enhanced TNF production in response to LPS by 6.6-fold compared to collagen-coated dishes.

Conclusions:

  • Colonic macrophages possess an inherent ability to produce TNF.
  • Activation by specific, potentially unknown, substances may trigger TNF production in intestinal macrophages.
  • This induced TNF production could contribute to the intestinal inflammation observed in Crohn's disease.

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