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Expression of interferon inducible protein-10 in pancreas of mice
Dong Li1, Su-Wen Zhu, Dong-Juan Liu
1Department of Infectious Disease, the First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China. ldcmu@yahoo.com.cn
Aim:
To investigate the expression of interferon inducible protein-10 (IP-10) in pancreas of mice and to discuss its possible role in the pathogenesis of type 1 diabetes.
Methods:
Non-obese diabetic (NOD) mice were used as experiment group and BALB/c mice as non-diabetic prone model. Immunohistochemistry method was used to evaluate the expression of IP-10 in the pancreas of NOD mice and BALB/c mice. Immunoelectron microscope was used to show the location of IP-10 in pancreatic islet beta cells.
Results:
Pancreatic islets were positively stained in all the NOD mice. Insulitis could be found in mice at the age of 4 wk. The weakly positive results were found in control group with no insulitis. Immunoelectron microscopy further demonstrated that IP-10 was produced by pancreatic beta cells and stored in cytoplasm of the cells.
Conclusion:
IP-10 can be largely produced in pancreatic islets of NOD mice at the age of 2 wk when there is no significant insulitis, and may play an important part in the pathogenesis of type 1 diabetes by attracting immune cells to infiltrate the pancreatic islets.
