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Which short tandem repeat polymorphisms are required for identification? Lessons from complicated kinship cases.
1Institut für Blutgruppenforschung, Hohenzollernring 57, 50672 Köln, Germany. D49221253037@t-online.de
Croatian Medical Journal
|August 16, 2005
Summary
Extensive DNA typing using short tandem repeat (STR) and restriction fragment length polymorphisms (RFLPs) resolved complex parentage cases. New STR systems are needed to replace RFLPs as they become obsolete.
Area of Science:
- Forensic Genetics
- Molecular Biology
Background:
- Deficient parentage cases present unique challenges in genetic identification.
- Short tandem repeat (STR) and restriction fragment length polymorphisms (RFLPs) are established DNA typing methods.
Purpose of the Study:
- To demonstrate the utility of extensive DNA typing in resolving complicated deficient parentage cases.
- To assess the future role of STR and RFLP techniques in forensic genetics.
Main Methods:
- Utilized extensive DNA typing, including STR and RFLP analysis.
- Applied these methods to five complex deficient parentage cases.
Main Results:
- Successfully resolved all five complicated deficient parentage cases through comprehensive DNA typing.
- RFLPs were effective but require high-quality DNA and are labor-intensive.
- RFLP technique is becoming less accessible, necessitating advanced STR systems.
Conclusions:
- Extensive DNA typing, particularly with STRs, is crucial for resolving complex parentage disputes.
- The development of highly polymorphic STR systems is essential to replace the declining use of RFLPs.
- Novel STR loci like ACTBP2 (SE33), D5S2360, gonosomal loci, and kits like "Humantype Chimera" show promise.