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20-Hydroxyeicosatetraenoic acid is excreted as a glucuronide conjugate in human urine
C Prakash1, J Y Zhang, J R Falck
1Department of Pharmacology, Vanderbilt University, Nashville, TN 37232.
Biochemical and Biophysical Research Communications
|June 15, 1992
Abstract:
20-hydroxyeicosatetraenoic acid, a major renal P-450 metabolite of arachidonic acid, has been quantified in human urine using capillary gas chromatography/electron capture negative ion chemical ionization mass spectrometry. The urinary excretion of 20-hydroxyeicosatetraenoic acid was in the low pg/ml range. However, treatment of urine with beta-glucuronidase resulted in a 13- to 28-fold increase in its concentration. This suggests 20-hydroxyeicosatetraenoic acid differs from other eicosanoids in that it is excreted primarily as a glucuronide conjugate.