Related Experiment Video

Updated: Aug 16, 2026

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

[Detergents for crystallization of membrane protein]

Shigeharu Harada1

  • 1harada@kit.ac.jp

Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme
|August 18, 2005
PubMed
Summary

No abstract available in PubMed .

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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...