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Optimal seeding conditions for human endothelial cells.

K C Kent1, A Oshima, A D Whittemore

  • 1Department of Surgery, Beth Israel Hospital, Boston, Massachusetts.

Annals of Vascular Surgery
|May 1, 1992
PubMed
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This study developed an in vitro model for arterial prosthesis seeding, finding plasma coating and a 90-minute incubation optimal for endothelial cell retention. This research aids in optimizing cell seeding protocols for clinical trials.

Area of Science:

  • Biomaterials Science
  • Vascular Surgery
  • Cell Biology

Background:

  • Arterial prostheses require endothelial cell seeding for improved patency.
  • Optimizing endothelial cell seeding is crucial for graft success.
  • Current seeding protocols lack detailed in vitro evaluation.

Purpose of the Study:

  • To develop and utilize an in vitro model for evaluating endothelial cell seeding on arterial prostheses.
  • To determine optimal graft coating, incubation time, and cell seeding density.
  • To inform the development of clinical in vivo seeding protocols.

Main Methods:

  • Development of an in vitro perfusion circuit model.
  • Radiolabeling of human saphenous vein endothelial cells with tritiated thymidine.

Related Experiment Videos

  • Seeding cells onto polytetrafluoroethylene grafts and assessing cellular retention under varying conditions (graft coating, incubation time, cell density).

    • Main Results:

    • Plasma coating demonstrated effectiveness comparable to fibronectin for enhancing endothelial cell retention.
    • Optimal seeding densities ranged from 1 to 2 x 10^5 cells/cm^2 for achieving a confluent monolayer.
    • A 90-minute incubation period significantly increased postperfusion cell attachment compared to shorter durations.

    Conclusions:

    • The developed in vitro model effectively evaluates key parameters of endothelial cell seeding on arterial prostheses.
    • Plasma coating and optimized incubation times/densities can improve cell retention.
    • Findings provide a basis for developing improved protocols for clinical in vivo seeding trials.