Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Factors affecting selectivity in ion chromatography.

James S Fritz1

  • 1Department of Chemistry, Iowa State University and Ames Laboratory, Ames, IA 50011-3111, USA. kniss@ameslab.gov

Journal of Chromatography. A
|August 19, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery and early development of non-suppressed ion chromatography.

Journal of chromatographic science·2010
Same author

A rapid, simple method for determining formaldehyde in drinking water using colorimetric-solid phase extraction.

Talanta·2008
Same author

Separation of organic cations using novel background electrolytes by capillary electrophoresis.

Journal of chromatography. A·2008
Same author

Determination of glycerophosphate and other anions in dentifrices by ion chromatography.

Journal of chromatography. A·2006
Same author

Single-column method of ion chromatography for the determination of common cations and some transition metals.

Journal of chromatography. A·2006
Same author

Ultrafast concentration and speciation of chromium(III) and (VI).

Journal of chromatography. A·2006
Same journal

Impurity profiling of lipid-conjugated oligonucleotides using reversed-phase with and without ion-pair reagents and hydrophilic interaction liquid chromatography.

Journal of chromatography. A·2026
Same journal

Preparation of magnetic zwitterionic covalent organic frameworks for rapid simultaneous extraction of hydrophilic and hydrophobic organophosphates from environmental waters coupled with UHPLC-MS/MS determination.

Journal of chromatography. A·2026
Same journal

Analysis of organic and inorganic acids in biomass pyrolysis process samples by ion chromatography-mass spectrometry.

Journal of chromatography. A·2026
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
Same journal

Ion chromatography: Current strengths, key limitations, and future trends.

Journal of chromatography. A·2026
See all related articles

Ion chromatography methods rely on cation-anion pair formation in the stationary phase. Selectivity is influenced by electrostatic and enforced-pairing interactions, affecting ion separation mechanisms.

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Ion chromatography methods like ion-exchange, ion-pair, and zwitterion chromatography share a common principle: cation-anion pair formation within the stationary phase.
  • Selectivity in these techniques is determined by the sample ions' ability to form these pairs, influenced by stationary phase properties and solvation.

Purpose of the Study:

  • To define selectivity in ion chromatography based on cation-anion pair formation.
  • To explore the impact of stationary phase characteristics and mobile phase composition on ion separation and elution order.
  • To elucidate the separation mechanism in zwitterion ion chromatography.

Main Methods:

  • Anion-exchange chromatography was used to demonstrate the effect of resin cation properties (geometry, bulkiness, polarity) on selectivity.

Related Experiment Videos

  • The influence of resin matrix type, surface hydrophobicity, and solvation degree on chromatographic behavior was investigated.
  • Analysis of selectivity series in ion chromatography, considering electrostatic attraction (ES) and enforced-pairing (EP) mechanisms.

    • Main Results:

    • Selectivity is governed by the interplay of electrostatic attraction and enforced-pairing (hydrophobic and water-enforced).
    • Ion-pair and zwitterion chromatography exhibit different anion elution orders compared to conventional ion-exchange chromatography due to mobile phase ion effects.
    • Zwitterion ion chromatography shows unique retention for small, doubly charged anions and allows elution with pure water.

    Conclusions:

    • The separation mechanism in zwitterion ion chromatography is likely a dominant partitioning mechanism, possibly with a minor ion-exchange component.
    • Understanding the factors influencing cation-anion pair formation is crucial for optimizing ion separation in various chromatography techniques.
    • Zwitterion ion chromatography offers distinct advantages, including simplified elution and detection methods.