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Related Concept Videos

Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

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Updated: Jun 27, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

Structural domains in RNAi.

Robert E Collins1, Xiaodong Cheng

  • 1Department of Biochemistry, Emory University, 1510 Clifton Road, Atlanta, GA 30322, USA.

FEBS Letters
|August 19, 2005
PubMed
Summary
This summary is machine-generated.

RNA silencing involves precise double-stranded RNA recognition and processing for RISC complex formation. Recent structural studies reveal the molecular mechanisms of Drosha, Dicer, and RISC in this essential pathway.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • RNA silencing is a crucial biological process regulating gene expression.
  • The formation of the RNA-induced silencing complex (RISC) is central to this pathway.
  • Precise recognition and processing of double-stranded RNA (dsRNA) are key features of RNA silencing.

Purpose of the Study:

  • To review recent structural data elucidating RNA silencing mechanisms.
  • To highlight the molecular basis of dsRNA recognition and processing.
  • To present models of key protein functions, including Drosha, Dicer, and RISC.

Main Methods:

  • Review of published structural and biochemical studies.
  • Analysis of molecular structures of key RNA silencing proteins.
  • Integration of structural data with biochemical findings to model mechanisms.

Main Results:

  • Recent structures provide insights into the precise binding and cleavage of dsRNA.
  • Models illustrate how Drosha and Dicer process precursor RNAs.
  • Structural data informs the mechanism of RISC assembly and function.

Conclusions:

  • Structural biology is critical for understanding RNA silencing pathways.
  • Precise dsRNA processing by Drosha and Dicer is essential for RISC formation.
  • Ongoing structural and biochemical studies continue to refine models of RNA silencing.