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Activating E2Fs mediate transcriptional regulation of human E2F6 repressor
Tarrah E Lyons1, Maysoon Salih, Balwant S Tuana
1Dept. of Cellular and Molecular Medicine, Faculty of Medicine, Univ. of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada K1H 8M5.
American Journal of Physiology. Cell Physiology
|August 19, 2005
Summary
The E2F6 repressor
Area of Science:
- Molecular Biology
- Cell Cycle Regulation
Background:
- E2F6 is a transcription factor that represses E2F-responsive genes.
- Its role in cell cycle regulation is under investigation.
Purpose of the Study:
- To investigate the transcriptional regulation of E2F6.
- To determine the role of E2F family members in controlling E2F6 expression.
Main Methods:
- Analysis of the human E2F6 promoter region.
- Site-directed mutagenesis and deletion studies.
- Ectopic expression of E2F1.
- Electrophoretic mobility shift assays (EMSAs).
- Chromatin immunoprecipitation (ChIP) assays.
Main Results:
- The E2F6 promoter contains two critical E2F binding sites.
- E2F1 upregulates E2F6 mRNA and promoter activity.
- E2F1 binds to the E2F6 promoter in vitro and in vivo.
- E2F1 and other activating E2Fs are induced before E2F6 during the G1/S phase transition.
Conclusions:
- E2F6 expression is regulated transcriptionally by E2F family members.
- E2F1 plays a significant role in upregulating E2F6.
- Interplay among E2Fs, particularly E2F1, is crucial for cell cycle control.