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Related Experiment Videos

A temperature-jump device for time-resolved cryo-transmission electron microscopy.

M H Chestnut1, D P Siegel, J L Burns

  • 1Procter and Gamble Company, Cincinnati, OH 45239-8707.

Microscopy Research and Technique
|January 1, 1992
PubMed
Summary

A new temperature-jump device enables time-resolved studies of cryo-transmission electron microscopy specimens. Rapid heating induces microstructural changes before cryo-fixation, preserving dynamic processes.

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Area of Science:

  • Materials Science
  • Biophysics
  • Electron Microscopy

Background:

  • Cryo-transmission electron microscopy (cryo-TEM) is crucial for visualizing biological and chemical structures at near-native states.
  • Studying dynamic structural changes requires rapid fixation methods to capture transient states.
  • Existing methods may not adequately capture rapid thermal transitions in thin specimens.

Purpose of the Study:

  • To develop and validate a novel temperature-jump device for time-resolved cryo-TEM.
  • To enable the study of microstructural dynamics induced by rapid heating.
  • To assess the effectiveness of rapid cryo-fixation following thermal transitions.

Main Methods:

  • A xenon arc lamp and timing circuitry were used for rapid specimen heating.

Related Experiment Videos

  • An environmental specimen preparation chamber facilitated controlled heating and plunging.
  • A thermocouple integrated with an electron microscope grid measured temperature changes.
  • Dimyristoyl phosphatidylcholine (DMPC) vesicles and n-docosane films were used as model systems.

    • Main Results:

    • Temperature jumps of 30-60 K were achieved within 150-450 milliseconds exposure times.
    • Specimens remained 20-30 K above initial temperatures upon contact with cryogen.
    • Micrographs confirmed the preservation of structural states after rapid heating and fixation.

    Conclusions:

    • The developed temperature-jump device effectively permits time-resolved studies of thin cryo-TEM specimens.
    • This technique allows for the capture of microstructural changes induced by rapid temperature increases.
    • The method is applicable to diverse biological and chemical systems exhibiting temperature-activated structural dynamics.