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Complexes between anionic liposomes and spherical polycationic brushes. An assembly of assemblies.

A V Sybachin1, O V Zaborova, V N Orlov

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This study demonstrates assembling anionic liposomes onto spherical polycationic brushes (SPBs) to create high-capacity nanoparticles. The resulting complexes show tunable charge, aggregation behavior, and liposome stability, with potential applications in drug delivery.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biophysics

Background:

  • Developing nanoparticles with high loading capacity is crucial for applications like drug delivery.
  • Spherical polycationic brushes (SPBs) offer a versatile platform for nanoparticle functionalization.
  • Anionic liposomes are key components for targeted delivery and controlled release.

Purpose of the Study:

  • To synthesize and characterize nanoparticle-liposome complexes with high loading capacity.
  • To investigate the effect of liposome composition on complex formation and stability.
  • To explore the charge, aggregation, and dissociation properties of these novel assemblies.

Main Methods:

  • Synthesis of spherical polycationic brushes (SPBs) via graft polymerization.
  • Complexation of anionic liposomes (egg lecithin and phosphatidylserine) with SPBs at varying ratios (ν).
  • Characterization using electrophoretic mobility, dynamic light scattering, conductivity, fluorescence, and cryogenic transmission electron microscopy.

Main Results:

  • Liposomes fully associate with SPBs up to a saturation concentration, with the number of liposomes per SPB varying with ν.
  • SPB complexes exhibit charge reversal at high liposome concentrations and aggregate upon charge neutralization.
  • Liposomes remain intact, and their association with SPBs is dependent on liposome composition and salt concentration, with some complexes showing high stability.

Conclusions:

  • SPB-liposome complexes can be formed with high loading capacities and tunable properties.
  • Liposome composition significantly influences complex stability and dissociation behavior.
  • The study provides insights into the self-assembly of charged nanoparticles and liposomes, relevant for advanced material design.