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Related Experiment Videos

Cyclical interactions between two outer doublet microtubules in split flagellar axonemes.

Susumu Aoyama1, Ritsu Kamiya

  • 1Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Biophysical Journal
|August 23, 2005
PubMed
Summary

The mechanism of cilia and flagella beating involves microtubule sliding. This study observed microtubule dynamics in Chlamydomonas axonemes, proposing a new model for how bending is generated.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Molecular Motors

Background:

  • Cilia and flagella generate movement through the coordinated sliding of outer doublet microtubules.
  • The precise mechanism driving the oscillatory bending of these structures remains poorly understood.

Purpose of the Study:

  • To investigate the dynamic interactions between microtubules in the axoneme.
  • To elucidate the mechanism responsible for oscillatory bending in cilia and flagella.

Main Methods:

  • High-speed video recording of frayed Chlamydomonas axonemes.
  • Observation of microtubule-dynein interactions in the presence of ATP.

Main Results:

  • Adjacent doublet microtubules exhibited cycles of association and dissociation.

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  • Microtubule dissociation often occurred without significant bending, challenging curvature-dependent regulation models.
  • Simultaneous association and dissociation in a single pair led to tip-directed gap movement.

    • Conclusions:

    • A novel model for cyclical bend propagation in axonemes is proposed.
    • Dynein-microtubule interactions may not be solely regulated by microtubule curvature.
    • Understanding these dynamics is crucial for cilia and flagella function.