Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basic investigations in Saccharomyces cerevisiae.

Brendan P G Curran1, Virginia Bugeja

  • 1School of Biological Sciences at Queen Mary, University of London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2005
PubMed
Summary

This chapter compiles essential laboratory techniques for working with yeast cells. It covers methods for growing, storing, mating, and sporulating yeast. The text includes recipes for media and step-by-step protocols for genetic crosses and spore isolation. It also introduces online resources for yeast research. The goal is to provide a single reference for researchers at all levels. The chapter emphasizes standardized procedures to ensure reproducibility. It addresses the need for accessible and detailed laboratory methods in yeast biology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Basic investigations in Saccharomyces cerevisiae.

Methods in molecular biology (Clifton, N.J.)·2014
Same author

Differential effects of hydrogen peroxide and ascorbic acid on the aerobic thermosensitivity of yeast cells grown under aerobic and anoxic conditions.

Yeast (Chichester, England)·2009
Same author

Can the different heat shock response thresholds found in fermenting and respiring yeast cells be attributed to their differential redox states?

Yeast (Chichester, England)·2007
Same author

Reactive oxygen species may influence the heat shock response and stress tolerance in the yeast Saccharomyces cerevisiae.

Yeast (Chichester, England)·2004
Same author

Dioctyl phthalate increases the percentage of unsaturated fatty acids with a concomitant decrease in cellular heat shock sensitivity in the yeast Saccharomyces cerevisiae.

Microbiology (Reading, England)·2000
Same author

Cellular lipid composition influences stress activation of the yeast general stress response element (STRE).

Microbiology (Reading, England)·2000

Area of Science:

  • Molecular genetics
  • Cell biology
  • Microbial physiology

Background:

Prior research has established standard methods for cultivating and manipulating yeast cells. These include media preparation, growth protocols, and storage techniques. However, a comprehensive guide integrating these procedures is still lacking. No prior work has resolved the need for a single reference covering all basic yeast lab techniques. This gap motivated the compilation of standardized protocols. Researchers have shown that yeast remains a model organism for genetic and cellular studies. Yet, detailed instructions for mating and sporulation are not widely accessible. This paper addresses the need for a consolidated resource.

Purpose Of The Study:

The aim of this chapter is to compile essential methods for working with yeast cells. It focuses on procedures for growth, storage, mating, and sporulation. The study seeks to provide a single reference for laboratory techniques. It addresses the need for standardized protocols in yeast research. The motivation stems from the increasing importance of yeast in post-genomic studies. The chapter seeks to simplify complex procedures like spore isolation. It also aims to highlight online resources for yeast research. This compilation supports both novice and experienced researchers.

Keywords:
yeast research protocolsgenetic cross setupSaccharomyces cerevisiae methodspost-genomic yeast studies

Frequently Asked Questions

The authors propose that the chapter compiles essential methods for yeast research, including growth, storage, mating, and sporulation.

The chapter provides detailed protocols for both short- and long-term yeast cell storage, ensuring viability and reproducibility.

The authors suggest that spore isolation requires precise conditions and careful handling to ensure successful sporulation and spore viability.

The chapter introduces Internet-based yeast resources as essential tools for post-genomic research, aiding in data interpretation and analysis.

Related Experiment Videos

Main Methods:

The chapter begins with recipes for various yeast media. It outlines steps for growing cells at specific stages of the growth cycle. Detailed protocols for short- and long-term storage are included. The text explains how to set up genetic crosses between yeast strains. It provides instructions for diploid cell sporulation and spore isolation. These methods are based on established laboratory practices. The chapter also introduces online resources for yeast research. All procedures are designed for reproducibility and ease of use.

Main Results:

The chapter successfully compiles a range of yeast laboratory techniques. It provides detailed media recipes and growth protocols. Storage methods for both short- and long-term use are clearly outlined. Genetic cross setups are explained with step-by-step instructions. Sporulation and spore isolation techniques are described in detail. Online resources are highlighted as essential tools for modern yeast research. The compilation ensures that all procedures are accessible and reproducible. This resource fills a gap in the literature for basic yeast methods.

Conclusions:

The authors propose that this chapter serves as a comprehensive guide for yeast research. It synthesizes established methods into a single reference. The chapter emphasizes the importance of standardized protocols. It highlights the role of online resources in post-genomic yeast studies. The authors suggest that this compilation supports both new and experienced researchers. The text provides a framework for reproducible experiments in yeast biology. It addresses the need for accessible and detailed laboratory procedures. This resource enhances the practical utility of yeast as a model organism.

Genetic crosses allow researchers to study inheritance patterns and gene function, as outlined in the chapter's detailed setup instructions.

The authors propose that the chapter provides a consolidated reference for basic yeast methods, enhancing reproducibility and accessibility.