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Basic investigations in Saccharomyces cerevisiae.

Brendan P G Curran1, Virginia Bugeja

  • 1School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, E1 4NS, UK, B.curran@qmul.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|May 21, 2014
PubMed
Summary

This guide offers essential yeast cell techniques, covering cultivation, storage, mating, and sporulation for Saccharomyces cerevisiae research. It highlights web resources and genetic crossing methods for post-genomic studies.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Yeast (Saccharomyces cerevisiae) is a model organism in biological research.
  • Post-genomic era research increasingly relies on web-based resources for yeast investigation.
  • Standardized protocols are crucial for reproducible yeast cell manipulation.

Purpose of the Study:

  • To serve as a comprehensive reference for fundamental yeast cell procedures.
  • To guide researchers in accessing, cultivating, storing, mating, and sporulating yeast.
  • To introduce essential web-based resources for Saccharomyces cerevisiae research.

Main Methods:

  • Detailed recipes for various yeast growth media.
  • Protocols for achieving optimal cell numbers and growth cycle stages.

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  • Methods for both short-term and long-term yeast cell storage.
  • Techniques for setting up genetic crosses, including diploid cell sporulation and spore isolation.

    • Main Results:

    • Provides accessible information on yeast cell culture and maintenance.
    • Enables researchers to perform genetic crosses and sporulation efficiently.
    • Integrates the use of web-based resources for yeast research.

    Conclusions:

    • This chapter equips researchers with the necessary skills for comprehensive yeast cell management.
    • Facilitates advanced studies in Saccharomyces cerevisiae using established and novel techniques.
    • Serves as a valuable resource for both novice and experienced yeast researchers.