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Basic investigations in Saccharomyces cerevisiae.

Brendan P G Curran1, Virginia Bugeja

  • 1School of Biological Sciences at Queen Mary, University of London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2005
PubMed
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This guide details essential techniques for yeast cell culture, including growth, storage, mating, and sporulation. It covers media preparation, cell cycle synchronization, genetic crosses, and diploid sporulation for Saccharomyces cerevisiae research.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Standardized protocols are crucial for reproducible yeast research.
  • Saccharomyces cerevisiae is a key model organism in molecular biology and genetics.
  • Post-genomic research necessitates efficient methods for yeast manipulation.

Purpose of the Study:

  • To offer a comprehensive guide to fundamental yeast cell handling techniques.
  • To detail procedures for yeast cultivation, preservation, reproduction, and sporulation.
  • To introduce valuable online resources for Saccharomyces cerevisiae research.

Main Methods:

  • Detailed recipes for various yeast growth media.
  • Step-by-step protocols for cell culturing and synchronization.

Related Experiment Videos

  • Methods for short- and long-term yeast cell storage.
  • Techniques for setting up genetic crosses.
  • Procedures for diploid cell sporulation and spore isolation.

    • Main Results:

    • Established protocols for yeast growth, storage, mating, and sporulation.
    • Guidance on achieving appropriate cell numbers and growth cycle stages.
    • Comprehensive explanation of genetic crossing and sporulation techniques.
    • Introduction to essential internet-based resources for yeast research.

    Conclusions:

    • This chapter serves as a vital reference for researchers working with yeast.
    • The provided methods facilitate efficient manipulation of Saccharomyces cerevisiae.
    • Access to online resources enhances post-genomic investigations of yeast.