Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Yeast two-hybrid system screening.

R Daniel Gietz1

  • 1Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Yeast transformation by the LiAc/SS carrier DNA/PEG method.

Methods in molecular biology (Clifton, N.J.)·2014
Same author

Yeast transformation by the LiAc/SS carrier DNA/PEG method.

Methods in molecular biology (Clifton, N.J.)·2014
Same author

Yeast two-hybrid liquid screening.

Methods in molecular biology (Clifton, N.J.)·2014
Same author

Chemical reactivity and microbicidal action of bethoxazin.

Bioorganic & medicinal chemistry·2012
Same author

Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method.

Nature protocols·2007
Same author

Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method.

Nature protocols·2007
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This guide details the yeast two-hybrid system, a powerful genetic tool for identifying gene interactions. It provides comprehensive methods for researchers to perform and analyze yeast two-hybrid screens for their gene of interest.

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • The yeast two-hybrid system is a widely used molecular genetic technique.
  • It enables the study of protein-protein interactions in vivo.

Purpose of the Study:

  • To provide a comprehensive set of methods for conducting a yeast two-hybrid screen.
  • To guide researchers through the preparation, execution, and analysis of yeast two-hybrid experiments for their favorite gene (YFG).

Main Methods:

  • Detailed protocols for preparing a yeast two-hybrid screen.
  • Procedures for performing the actual yeast two-hybrid screen.
  • Methods for analyzing positive results obtained from the screen.

Main Results:

  • The article serves as a practical manual for implementing the yeast two-hybrid system.

Related Experiment Videos

  • Readers will gain the necessary skills to successfully perform yeast two-hybrid screens.

    • Conclusions:

    • The provided methods empower researchers to effectively utilize the yeast two-hybrid system for their specific research needs.
    • Successful negotiation of yeast two-hybrid screens is achievable using the described protocols.