Related Experiment Video
Updated: Aug 16, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
Published on: October 25, 2018
Gene expression profiling in single cells within tissue
Paola Capodieci1, Michael Donovan, Heidi Buchinsky
1Aureon Laboratories, 28 Wells Avenue, Yonkers, New York 10701, USA. paola.capodieci@aureon.com
Abstract:
We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.
Related Concept Videos
Cell Specific Gene Expression
Cell Specific Gene Expression
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
DNA Microarrays

