Related Experiment Video
Updated: Aug 16, 2026
Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test
Published on: July 27, 2015
The effects of interelectrode distance on electromyographic amplitude and mean power frequency during incremental
Moh H Malek1, Terry J Housh, Jared W Coburn
1Human Performance Laboratory, Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, 110 Ruth Leverton Hall, Lincoln, NE 68583-0806, USA. mmalek@unlserve.unl.edu
Abstract:
The purpose of this study was to examine the effects of interelectrode distance (IED) on the relationships of absolute and normalized EMG amplitude and mean power frequency (MPF) versus power output during incremental cycle ergometry. Eleven adults (mean +/- S.D. age = 24.2 +/- 2.6 y; V(O2max) = 49.4 +/- 8.3 ml kg(-1) min(-1)) performed incremental cycle ergometry tests. Surface EMG signals were recorded simultaneously from bipolar electrode arrangements placed over the VL muscle with IEDs of 20, 40, and 60 mm. Polynomial regression analyses were used to describe the relationships for absolute and normalized EMG amplitude (muV(rms) and % max) and MPF (Hz and % max) versus power output (%max) for each subject at the three IEDs. In addition, separate one-way repeated measures ANOVAs were used to examine mean differences between the three IEDs for absolute and normalized EMG amplitude and MPF at power outputs of 80, 110, 140, and 170 W. The results of the polynomial regression revealed that the best fit model for each IED for the absolute and normalized EMG amplitude was linear for six of the 11 subjects and quadratic for five of the subjects. For EMG MPF, four of the 11 subjects exhibited significant relationships (linear or quadratic) across power outputs for at least one IED. The one-way repeated measures ANOVAs revealed significant mean differences between the IEDs for absolute EMG amplitude and MPF at 80, 110, 140, and 170 W. There were no significant mean differences, however, between the IEDs for normalized EMG amplitude or MPF at 80, 110, 140, and 170 W. The results of the study indicated that there were no consistent patterns of responses between individual subjects for EMG amplitude or MPF versus power output relationships for IEDs of 20, 40, and 60 mm during incremental cycle ergometry. The current findings supported the process of normalization for EMG amplitude and MPF data obtained during cycle ergometry when comparisons are made for different IEDs.
