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Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
Published on: March 10, 2017
Cloning and expression of Staphylococcus epidermidis urease gene sequences in Staphylococcus carnosus
L Grüter1, M Endres, S Gatermann
1Institut für Medizinische Mikrobiologie und Immunologie, Universität Hamburg, FRG.
FEMS Microbiology Letters
|May 15, 1992
Abstract:
The urease gene sequences of Staphylococcus epidermidis CNS23 were cloned and expressed in Staphylococcus carnosus TM300. In vitro translation of the cloned sequences revealed four polypeptides (60, 17, 11 and 7.5 kDa) that were associated with enzyme activity. Southern hybridisation experiments showed high homologies with the urease genes of Staphylococcus saprophyticus.

