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A conductive ormosil encapsulated with ferrocene conjugate and multiwall carbon nanotubes for biosensing application
Vivek Babu Kandimalla1, Vijay Shyam Tripathi, Huangxian Ju
1Key Laboratory of Analytical Chemistry for Life Science, Education Ministry of China, Department of Chemistry, Nanjing University, Nanjing 210093, China.
Biomaterials
|August 30, 2005
Summary
A novel ormosil composite film enhances glucose detection. This conductive, non-toxic biosensor utilizes ferrocenemonocarboxylic acid-bovine serum albumin conjugate and multiwall carbon nanotubes for improved electron transfer and enzyme stability.
Area of Science:
- Materials Science
- Electrochemistry
- Biotechnology
Background:
- Ormosil composite films offer tunable properties for biosensing.
- Developing stable and sensitive amperometric biosensors is crucial for glucose monitoring.
Purpose of the Study:
- To create a highly conductive and non-toxic ormosil composite film for glucose determination.
- To immobilize glucose oxidase (GOD) and a redox mediator for enhanced biosensor performance.
Main Methods:
- Preparation of an ormosil composite film using specific silanes.
- Doping the film with ferrocenemonocarboxylic acid-bovine serum albumin (FMC-BSA) conjugate and multiwall carbon nanotubes (MWNTs).
- Immobilization of glucose oxidase (GOD) for amperometric glucose detection.
Main Results:
- The prepared film exhibited excellent redox electrochemistry and high GOD stability.
- The biosensor achieved a detection limit of 20 microm (S/N=3) in the linear range of 0.05-20.0 mm.
- Flow system analysis time was 25 s per sample, with an apparent Michaelis constant (K(M)(app)) of 6.6 mm.
Conclusions:
- The proximity of FMC-BSA, MWNTs, and GOD enhanced electron transfer, leading to efficient biosensing.
- The developed film is suitable for entrapping other redox compounds and for broader biosensing applications.