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Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa
Published on: April 8, 2016
Strain variability in the DNA immigration control region (ICR) of Xylella fastidiosa
Simone Cristina Picchi1, Laurival Antonio Vilas-Boas, Paulo César Ceresini
1Departamento de Biologia Aplicada à Agropecuária, Faculdade de Ciências Agrárias e Veterinárias-FCAV/UNESP, Jaboticabal, SP, Brazil. mvictor@fcav.unesp.br
Abstract:
The genome of the bacterium Xylella fastidiosa contains four ORFs (XF2721, XF2725, XF2739 and XF0295) related to the restriction modification type I system, ordinarily named R-M. This system belongs to the DNA immigration control region (ICR). Each ORF is related to different operon structures, which are homologues among themselves and with subunit Hsd R from the endonuclease coding genes. In addition, these ORFs are highly homologous to genes in Pseudomonas aeruginosa, Methylococcus capsulatus str. Bath, Legionella pneumophila, Helicobacter pylori, Xanthomonas oryzae pv. Oryzae and Silicibacter pomeroyi, as well as to genes from X. fastidiosa strains that infect grapevine, almond and oleander plants. This study was carried out on R-M ORFs from forty-three X. fastidiosa strains isolated from citrus, coffee, grapevine, periwinkle, almond and plum trees, in order to assess the genetic diversity of these loci through PCR-RFLP. PCR-RFLP analysis of the four ORFs related to the R-M system from these strains enabled the detection of haplotypes for these loci. When the haplotypes were defined, wide genetic diversity and a large range of similar strains originating from different hosts were observed. This analysis also provided information indicating differences in population genetic structures, which led to detection of different levels of gene transfer among the groups of strains.
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