[Infrared spectra analysis of chromium cation biosorbed by biosorbent ZL5-2]

Biosorption of Cr( VI) by the novel biosorbent ZL5-2 produced by Agrobacterium. sp was studied, and infrared spectra of native, absorbing and desorbing ZL5-2 were compared. The initial pH was important for biosorption. The optimum effect on Cr(VI) biosorption was acquired within initial pH 0.5-1.5, then with the pH increasing, the effect on Cr6+ biosorption was reduced. The biosorption for Cr(VI) was a quick process, only within 10 min 65.4% Cr(VI) was biosorbed, and the biosorption was in equilibrium after 60 min, then all the Cr(VI) was biosorbed after 80 min. The absorbed Cr(VI) could be recovered by desorption, and the desorption rate reached 13.6%-67.9%. The absorbance peak around 3 400 cm(-1), which reflected OH stretching vibration, moved about 8 cm(-1), and its absorbance decreased; the absorbance peak around 2 900 cm(-1) decreased, which reflected C-H stretching vibration; and the absorbance peak around 1 600 cm(-1), which reflected amide I group stretching vibration, moved about 13 cm(-1), and its absorbance decreased. After desorption, the absorbance peaks which reflected OH and C-H were increased to the initial degree, and the absorbance peak which reflected NH and amide I group did not increase. Therefore, authors presumed that the process of biosorption was reversible adsorption and irreversible adsorption simultaneously, however the reversible adsorption was more important in the biosorption.