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Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
Javier García1, Alvaro Puentes, Luis Rodríguez
1Fundación Instituto de Inmunologia de Colombia and the Universidad Nacional de Colombia, Avda. Calle 26 No. 5000, Bogotá, Colombia 020304. javgar22@hotmail.com
Protein Science : a Publication of the Protein Society
|September 1, 2005
Summary
The Mycobacterium tuberculosis Rv2536 gene is present and transcribed in various strains. A specific peptide (11207) from this protein binds to human cell lines, indicating potential roles in host-pathogen interactions.
Area of Science:
- Microbiology
- Immunology
- Cell Biology
Background:
- The Rv2536 gene is part of the Mycobacterium tuberculosis complex.
- Understanding the function of Rv2536 is crucial for tuberculosis research.
Purpose of the Study:
- To investigate the presence and transcription of the Rv2536 gene in different Mycobacterium tuberculosis strains.
- To identify specific functional regions of the Rv2536 protein through peptide binding assays.
Main Methods:
- Polymerase Chain Reaction (PCR) and Reverse Transcription PCR (RT-PCR) were used to detect the gene.
- Synthetic peptides covering the Rv2536 sequence were used in binding assays with U937 and A549 cells.
- Antibody production in rabbits and cross-linking assays were employed to characterize peptide interactions.
Main Results:
- The Rv2536 gene was confirmed to be present and transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum.
- Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY180) showed high, saturable, nanomolar affinity binding to both U937 and A549 cells.
- Cross-linking assays identified specific binding of Peptide 11207 to 50 kDa U937 and 45 kDa A549 cell membrane proteins.
Conclusions:
- The Rv2536 protein is expressed across key Mycobacterium tuberculosis strains.
- A specific peptide derived from Rv2536 interacts with host cell membrane proteins, suggesting a role in host-pathogen interactions.