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Updated: Aug 16, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Relation between genotype of HBV and its clinical manifestation by PCR with genotype-specific primers]
Zhi-Li Wen1, De-Ming Tan, Zhou-Hua Hou
1Department of Epidemiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Objective:
To determine the relation between genotype of HBV and its clinical manifestation.
Methods:
Sera of 220 patients from Hunan Province of China, infected chronically with asymptomatic, mild, moderate, severe and fulminant hepatitis hepatitis B, were genotyped by PCR with genotype-specific primers. The results were statistically analyzed.
Results:
Only genotype B (86.4%) and C (13.6%) were found. With the aggravations of patients' condition, genotype C was found more often (P < 0.05). The levels of alanine aminotransferase (ALT), total bilirubin (TBIL) and HBV-DNA of genotype C was higher than those of genotype B, but there was no statistical difference. However, the ALT elevation rates of genotype C (96.7%) were significantly higher than those of genotype B (75.2%) (P < 0.05). There was no statistical difference in HBeAg rate between genotype B and C in general, but in patients with chronic fulminant hepatitis or aged 21 approximately 30, the HBeAg rates of genotype C (35.0% and 50.0%) were significantly higher than those of genotype B (14.4% and 24.5%) (P < 0.05).
Conclusion:
The dominant genotype of HBV in Hunan Province of China is genotype B and C. Genotype of HBV correlates with its clinical manifestation and genotype C of HBV can lead to more severe hepatitis than genotype B of HBV.
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