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A Place for RNAi

Paul Anderson1

  • 1Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.

Developmental Cell
|September 6, 2005
PubMed

Insights

Processing bodies (P bodies) are cellular structures involved in mRNA degradation. A newly identified protein in C. elegans appears to promote the assembly of these crucial P bodies.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Processing bodies (P bodies) are cytoplasmic foci central to mRNA degradation.
  • In mammalian cells, P bodies are linked to miRNA-mediated translational silencing and siRNA-induced mRNA decay.

Purpose of the Study:

  • To investigate the factors influencing P body assembly in C. elegans.
  • To identify novel proteins interacting with argonaute and affecting P body formation.

Main Methods:

  • Yeast three-hybrid screening to identify argonaute-interacting proteins.
  • RNA interference (RNAi) and immunofluorescence microscopy in C. elegans.

Main Results:

  • An argonaute-interacting protein was identified that promotes P body assembly.
  • Depletion of this protein leads to reduced P body formation in C. elegans.

Conclusions:

  • This argonaute-interacting protein plays a role in the regulation of P body biogenesis.
  • Findings provide new insights into the molecular mechanisms governing mRNA decay pathways.

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