Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell surface immunoglobulin regulated checkpoints in chicken B cell development.

Parinaz Aliahmad1, Kelly A Pike, Michael J H Ratcliffe

  • 1Department of Immunology, University of Toronto, 1 King's College Circle, Toronto, Ont., Canada M5S 1A8.

Veterinary Immunology and Immunopathology
|September 6, 2005
PubMed
Summary

Surface immunoglobulin M (sIgM) expression is crucial for B lymphocyte colonization in chick embryos. However, receptor ligation, not just expression, is needed for B cell development post-hatch.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First-In-Human Safety, Pharmacokinetics, and Pharmacodynamics of VENT-02 Freebase, a CNS-Penetrant NLRP3 Inhibitor.

Clinical and translational science·2026
Same author

Enhanced efficacy of a next-generation EEEV self-replicating RNA platform for combination cancer immunotherapies.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Reducing hERG Inhibition in the Design of Potent and Bioavailable Indazole cGAS Inhibitors.

ACS medicinal chemistry letters·2026
Same author

Durability of next-generation self-replicating RNA vaccine RBI-4000: a phase 1, randomized open label clinical trial.

Communications medicine·2025
Same author

Divergent Delivery and Expression Kinetics of Lipid and Polymeric Nanoparticles across mRNA Modalities.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Structural insight into the cGAS active site explains differences between therapeutically relevant species.

Communications chemistry·2025

Area of Science:

  • Immunology
  • Developmental Biology
  • Avian Biology

Background:

  • The bursa of Fabricius is essential for B lymphocyte development in birds.
  • B cell precursor colonization of bursal follicles requires surface immunoglobulin (sIg) expression.

Purpose of the Study:

  • To investigate the role of chimeric CD8-Ig receptors in B cell development.
  • To determine the requirements for bursal colonization and post-hatch development in chickens lacking endogenous sIgM.

Main Methods:

  • Retroviral gene transfer to express chimeric CD8-Ig receptors in chicken embryos.
  • Intrabursal administration of anti-CD8 antibodies to ligate chimeric receptors.
  • Analysis of bursal cell development and sIgM expression.

Related Experiment Videos

Main Results:

  • Chimeric CD8-Ig receptor expression supported bursal colonization in embryos lacking sIgM.
  • Chimeric receptor expression alone did not support B cell development after hatching.
  • Anti-CD8 antibody ligation of chimeric receptors maintained bursal cell numbers post-hatch in the absence of sIgM.

Conclusions:

  • sIgM receptor expression is necessary for productive bursal colonization in chick embryos.
  • sIgM receptor ligation is required for sustained B cell development after hatching.