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Updated: Aug 16, 2026

08:21
Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
Published on: January 14, 2021
Preparation, isolation and characterization of stage-specific spermatogenic cells for cellular and molecular analysis
Noora Kotaja1, Sarah Kimmins, Stefano Brancorsini
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS-INSERM, Université Louis Pasteur, B.P. 163, 67404 Illkirch, Strasbourg, France.
Nature Methods
|September 8, 2005
Abstract
No abstract available in PubMed .
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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
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